Journal: Biomolecules

Article Title: Hypoxia-Induced Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Regulate Macrophage Polarization and Enhance Angiogenesis to Promote Diabetic Wound Healing

doi: 10.3390/biom15111504

Figure Lengend Snippet: Anti-inflammatory and pro-angiogenic effects of hy-EVs in vitro. ( a ) Immunofluorescent staining of CD86 (red) and CD206 (green) and ( b ) quantitative analysis of their fluorescence intensities ( n = 3 per group). ( c ) ROS fluorescent staining of HSFs and HUVECs in different treatment groups and ( d ) quantitative analysis of their fluorescence intensities ( n = 3 per group). ( e ) Immunofluorescence staining of HIF-1α (green), VEGFA (green) and CD31 (red) expression in different treatment groups, and the nuclei were stained with DAPI (blue); ( f ) quantitative analysis of their fluorescence intensities ( n = 3 per group). ( g ) Western blot analysis of the expression levels of HIF-1α, VEGFA and CD31 in different treatment groups (original Western blot images can be found in ), and ( h ) their quantitative analysis ( n = 3 per group). Data are mean ± SD, *** p < 0.001. All p values were obtained by one-way ANOVA.

Article Snippet: N-EVs and hy-EVs were labeled with Cell Plasma Membrane Red Fluorescence Staining Kit (PKH26, Beyotime, Shanghai, China).

Techniques: In Vitro, Staining, Fluorescence, Immunofluorescence, Expressing, Western Blot

Journal: Scientific reports

Article Title: Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients.

doi: 10.1038/s41598-024-78497-x

Figure Lengend Snippet: Fig. 1. Examples of electropherograms of ccfDNA isolates obtained on the Bioanalyzer 2100 using High Sensitivity DNA Kit. Isolates of sample number 5 with the highest quantity of ccfDNA per mL of plasma obtained using all three methods are shown. Electropherogram corresponding to sample isolated by NucleoSpin cfDNA XS Kit (Macherey-Nagel, Düren, Germany) is shown in red, QIAamp® ccfDNA/RNA Kit (Qiagen, Hilden, Germany) is shown in blue, and QIAamp® Circulating Nucleic Acid Kit (Qiagen, Hilden, Germany) is shown in green. In addition to the peaks corresponding to the lower (35 bp) and upper (10,380 pb) markers, peaks corresponding to the mononucleosome (~ 165 bp) and dinucleosome (~ 320 bp) are also visible.

Article Snippet: The isolation of ccfDNA using the NucleoSpin® cfDNA XS Kit (Macherey-Nagel, Düren, Germany) was performed from a thawed 700 μL aliquot of plasma according to the manufacturer’s guidelines using the enclosed proteinase K and a final elution volume of 20 μL.

Techniques: Clinical Proteomics, Isolation